Publications with our direct contribution are noted in black, and highly relevant publications in the field are in italics.

Long-term obeticholic acid treatment is associated with improvements in collagen morphometry in patients with primary biliary cholangitis. Andreas E. Kremer, Christopher L. Bowlus, Pierre Bedossa, Albert Parés, Lisa M. Forman, Joost P.H. Drenth, Stephen Ryder, Luigi Terracciano, Yuying Jin, Alexander Liberman, Richard Pencek, Leigh MacConell, Paul J. Pockros

Presented at EASL 2019 - Poster here

Morphometric collagen analysis discerns anti-fibrotic effects of INT-767 and OCA in NASH mouse models using second harmonic generation imaging. Li Chen, Mathieu Petitjean, Luciano Adorini, Jonathan Roth

Presented at EASL 2019 - Abstract SAT-342 - Poster here.

The Glucocorticoid Antagonist ST001 Prevents Development of NASH and Improves Aspects of the Metabolic Syndrom in the DIAMOND Mouse Model. Rebecca Caffrey

Presented at EASL 2019 - Abstract PS-129. Poster under this link after EASL 2019

ASK1 Inhibition Reduces Progression of Liver Cirrhosis and Prevents Tumor Development in the BALS/c.Mdr2-/- Mouse Model of Biliary Fibrosis. G. Wei, P. An, K, Vaid, G. Budas, A. Mikels-Vigdal, M. Kuand, Y. Popov.

Presented at AASDL 2018. Poster here.

Quantitative Assessment of Liver Septal Fibrosis Severity Using Morphometric Analysis, Li Chen PhD., Anthony Azzara PhD., Mathieu Petitjean PhD., Presented at NASH-TAG 2018.

Poster here

Fully Automated Quantitative Assessment for NASH Using the Genesis(R)200 Imaging System, Stephen A. Harrison, MD, Zachary Goodman, MD, PhD, Katherine M. Cebe, MD, Lakshmi Alaparthi, Fanny Monge, Peter G. Traber, MD, Dean Tai, PhD. Presented at NASH-TAG 2018.

Poster here.

Second Harmonic Generation Reveals Subtle Fibrosis Differences in Adult and Pediatric Nonalcoholic Fatty Liver Disease. Feng Liu, MD, Jing-Min Zhao, MD, Hui-Ying Rao, MD, Wei-Miao Yu, MD, Wei Zhang, MD, Neil D. Theise, MD, Aileen Wee, MBBS, FRCPath, FRCPA, and Lai Wei, MD. Am J Clin Pathol. 2017 Nov 20;148(6):502-512. doi: 10.1093/ajcp/aqx104

Paper here

Second Harmonic Generation Imaging Microscopy quantifies and characterizes fibrosis in the bowell wall of ulcerative colitis resection - A pilot study. Florian Reider et all. To be presented at DDW 2018 - June 2-5 2018

Label-free Dynamic Full-Field OCT generates novels images for the study of Stromae: collagen(s) and immune systems cells are revealed in submicronic resolution. Claude Boccara and LLCTECH Inc, December 6th 2017

See case study (PDF Here)

New Standards in Fibrosis Quantification: the Value of Label-free Second Harmonic Generation. Li Chen, Mathieu Petitjean at the Anti-fibrotic Drug Development Forum, November 13-14, 2017, Boston USA 

See slides from the podium presentation here

See poster here

INT-767 improves histopathological features in a diet-induced ob/ob mouse model of biopsy-confirmed non-alcoholic steatohepatitis (NASH). Jonathan D Roth, Michael Feigh, Sanne S Veidal, Louise K D Fensholdt, Kristoffer T G Rigbolt, Henrik H Hansen, Li C Chen, Mathieu Petitjean, Weslyn W Friley, Niels Vrang, Jacob Jelsing and Mark Young. World J Gastroenterol 2018 January 14; 24(2): 195-210

Paper here

Detailed Analysis of the Antifibrotic effect of INT-767, a dual FXR/TGR5 agonist in an obese mouse model of diet-induced and biopsy-confirmed NASH. Sanne Skovgård Veidal, Michael Feigh, Henrik B Hansen, Jacob Jelsing, Niels Vrang, Mathieu Petitjean, Li Chen, Mark Young, Jonathan Roth presented at AASLD 2017, October 20-23 , 2017.

See poster here (Presidential Award)

Novel Methods in Imaging and Quantification of Skeletal Muscle Fibrosis Associated with Severe Muscular Dystrophy. David W. Hammers, Coar C. Coker, Li Chen, Mathieu Petitjean, Elizabeth R.  Barton, H.Lee Sweeney presented at Keystone Injury, Inflammation and Fibrosis, March 26-30 2017.

See poster picture here (poster PDF file to come)

Use of Second Harmonic Generation (SHG) and 2-Photon Emission Imaging to Quantify and Describe the Structure of Tissue Fibrosis in the Rat TNBS intestinal fibrosis model. Laura Johnson, Li.Chen, P. Higgings et all. Poster #2008 presented at Keystone Injury, Inflammation and Fibrosis, March 26-30 2017.

Poster Here

Concurrent Semi-Automated Quantification of Liver Fibrosis and Fat Using Label-free Second Harmonic Generation Imaging in Animal Models. M. Petitjean, J. Schwartz, Li Chen. Poster #3003 - presented at Keystone Injury, Inflammation and Fibrosis, March 26-30 2017

Poster Here

Quantification of Septa in Liver Fibrosis using Second Harmonic Generation Imaging. Li Chen, Mohammad Naser,  et all. Poster #1016 - presented at Keystone Injury, Inflammation and Fibrosis, March 26-30 2017

Poster Here

Systematic Quantification of Histologic Patterns Shows Accuracy in Reflecting Cirrhotic Remodeling (qCP index). Yan Wang et All. doi: 10.1111/jgh.13722

Poster Here

Comparative efficacy of INT-767 vs. Liraglutide in Gubra AMLN Mice. Jonathan Roth et All. Intercept Pharmaceuticals. Keystone Symposia Workshop: approaches to NASH Treatment, March 7th 2017.

Paper Here

Dual Photon Microscopy Based Quantification of Fibrosis-related parameters (q-FP) to Model Disease Progression in Steatohepatitis (NASH). Yan Wang et all. Journal of Hepatology, 10.1002/hep.29090

Paper here

Automated evaluation of liver fibrosis in CCl4, BDL and TAA rodent models using Second Harmonic Generation / Two Photon excited fluorescence microscopy. Feng Liu et al. Laboratory Investigation (2016) , 1-9. 2016 USCAP

Paper here

New Classification of Liver Biopsy Assessment for Fibrosis in Chronic Hepatitis B Patients Before and After Treatment. Yameng Sun et all. Journal of Hepatology, doi: 10.1002/hep.29009

Paper here

Second Harmonic Generation microscopy: a novel automated method for quantification of hepatic steatosis in chronic liver disease. George B. Goh, Wei Qiang Leow, Shen Liang, Wei Keat Wan, Dean C. Tai, Kiat Hon Lim, Chee Kiat Tan, Jason P. Chang Poster # 1518 at AASLD 2016.

Read the poster here 

Development and validation of an automated optical imaging system to differentiate severity of hepatitis B- and non-alcoholic steatohepatitis-related fibrosis in patients with concomitant disease. Jason P. Chang, George B. Goh, Wei Qiang Leow, Wei Keat Wan, Dean C. Tai, Kiat Hon Lim, Chee Kiat Tan, Poster # 407 at AASLC 2016.

Read the poster here (soon)

Objective quantitative evaluation of anti-fibrotic agents in animal models. Kenneth Zhang, Li Chen, Bertha Xue, Chen Hu, Laurent Gole, and Chuan-Chu Chou. Poster # 69 at The First International Conference on Tissue Repair, Regeneration , and Fibrosis, September 21-25, 2016

See the Poster presentation here.

Stain-free detection and quantification of Bone Marrow Fibrosis using two-photon excitation and second harmonic generation microscopy. Leslie R. Rowe, Ali etman, Jessica Kohan, Josef Prchat, Gideon Ho, Dean Tai, Dong Quan Liu, Mohamed E. Salma at the 2015 US CAP Annual Conference, Poster #287 

Read the poster here

Digital Whole Slide Imaging and Two Photon Microscopy for Liver Fibrosis Scoring and Quantification. Jolanta Jedrzkiewicz, Mary P Bronner, Erinn Downs-Kelly, Jessica Kohan1, Leslie Rowe, Dean Tai, and Mohamed Salama at the 2015 US CAP Annual Conference, Poster #287 

Read the poster here

Automated biphasic morphological assessment of hepatitis B-related liver fibrosis using second harmonic generation microscopy (N=175). Tong-Hong Wang, Tse-Ching Chen, Xiao Teng, Kung-Hao Liang & Chau-Ting Yeh.  5:12962 | DOI : 10.1038/srep12962   

Read the article here.

qFibrosis: A fully-quantitative innovative method incorporating histological features to facilitate accurate fibrosis scoring in animal model and chronic hepatitis B patients. Shuoyu Xu, Yan Wang, Dean C.S. Tai, Shi Wang, Chee Leong Cheng, Qiwen Peng, Jie Yan, Yongpeng Chen, Jian Sun, Xieer Liang, Youfu Zhu, Jagath C. Rajapakse, Roy E. Welsch, Peter T.C. So, Aileen Wee, Jinlin Hou, Hanry Yu. Journal of Hepatology 2014 vol. 61 pages 260–269

Read the article here.

Fibro-C-Index: comprehensive, morphology-based quantification of liver fibrosis using second harmonic generation and two-photon microscopy. Dean C.S. Tai, Nancy Tan, Shuoyu Xu, Chiang Huen Kang, Ser Mien Chia, Chee Leong Cheng, Aileen Wee, Chiang Li Wei, Anju Mythreyi Raja, Guangfa Xiao, Shi Chang, Jagath C. Rajapakse. Journal of Biomedical Optics 14(4), 0440103 (July/August 2009)

Read the article here.

Nonlinear optical microscopy: use of second harmonic generation and two-photon microscopy for automated quantitative liver fibrosis studies. Sun W1, Chang S, Tai DC, Tan N, Xiao G, Tang H, Yu H. Journal of Biomedical Optics 13(6), 064010 (November/December 2008)

Read the article here.


Light-CT Scanner

Modified full-field optical coherence tomography: A novel tool for rapid histology of tissues. Manu Jain, Nidhi Shukla, Maryem Manzoor, Sylvie Nadolny, Sushmita Mukherjee. Journal of Pathology Informatics 2011; 2:28.

Read the article here.

Fibrosis impacts human adipocyte functions through mechanical cues. Pellegrinelli V, Heuvingh J, du Roure O, Rouault C, Devulder A, Klein C, Lacasa M, Clément E, Lacasa D and Clément K. Journal of Pathology 2014 Jun;233(2):183-95

Read the article here.